Analysis is performed using Local Run Manager or BaseSpace Sequence Hub.įor information on the performance of AmpliSeq for Illumina panels, see the data sheets listed on each of the AmpliSeq for Illumina product pages. Quality scores are measured with Q30 on Illumina systems. Paired-end sequencing facilitates detection of genomic rearrangements and repetitive sequence elements, as well as gene fusions and novel transcripts. Paired-end sequencing allows users to sequence both ends of a fragment and generate alignable sequence data. For guidance concerning the best fit for your experimental needs, refer to the AmpliSeq for Illumina flyer. However, due to per-sample output requirements, benchtop systems are typically the most appropriate. Contact Illumina Technical Support or your local Illumina representative for more assistance with making this decision.Īll Illumina systems can run AmpliSeq for Illumina. Comparing Illumina Sequencing Systemsįor a comparison of Illumina sequencing systems, see the Sequencing Platform Comparison Tool. Contact your local Territory Account Manager for assistance. ![]() Sequencing costs vary by system, throughput, and the number of samples per run. The equipment needed for performing an AmpliSeq for Illumina library prep is the same as the equipment needed for performing an AmpliSeq library experiment on the Ion Torrent.įor a list of required consumables and equipment, see AmpliSeq for Illumina Custom and Community Panels Consumables & Equipment. ![]() Illumina benchtop sequencing consumables are single cartridge-based with a separate flow cell. Ion PGM has accessory equipment that might no longer be needed. There are several additional training options available as well. ![]() These trainings go over experimental design, instrument operation, best practices, and troubleshooting. Onsite installation training from Field Application Scientists (FAS) is included with all benchtop system purchases except the iSeq 100 System. This resource provides step-by-step guidance through the process of transitioning from Ion Torrent to Illumina next-generation sequencing platforms.
0 Comments
Leave a Reply. |